Clonality:

Monoclonal

Synonyms:

HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

Host:

Mouse

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC, IP

Background:

Mouse monoclonal antibody to Histone H3 (DiMethyl K27)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human Histone H3. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/3000), IH (1/200 - 1/500), IP (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of Histone H3 (DiMethyl K27) expression in Hela (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of Histone H3 (DiMethyl K27) staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

Immunoprecipitation of Histone H3 (DiMethyl K27) from 0.5mg Hela whole cell extract lysate, using anti-Histone H3 (DiMethyl K27) Antibody.

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.