Clonality:

Monoclonal

Synonyms:

SCL; Keratin type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7

Host:

Mouse

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC, IP

Background:

Mouse monoclonal antibody to Cytokeratin 7

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of human Cytokeratin 7. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/100 - 1/200), IP (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of Cytokeratin 7 expression in Hela (A), mouse kidney (B), mouse brain (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of Cytokeratin 7 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

Immunoprecipitation of Cytokeratin 7 from 0.5mg Hela whole cell extract lysate, using anti-Cytokeratin 7 Antibody.

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.