Clonality:

Polyclonal

Synonyms:

RNF85; TNF receptor-associated factor 6; E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer; RING finger protein 85

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Pig

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to TRAF6

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRAF6. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of TRAF6 expression in Hela (A), K562 (B), Rat kidney (C), Rat heart (D), mouse kidney (E) whole cell lysates.

IHC description:

Immunohistochemical analysis of TRAF6 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.