Clonality:

Polyclonal

Synonyms:

TIM3; TIMD3; Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3

Host:

Rabbit

Reactivity:

Human, Mouse

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to TIM3

Immunogen:

KLH-conjugated synthetic peptide of human TIM3

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of TIM3 expression in HepG2 (A), BT474 (B), mouse lung (C), mouse thymus (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of TIM3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.