Clonality:

Polyclonal

Synonyms:

SEMAB; SEMB; Semaphorin-4A; Semaphorin-B; Sema B

Host:

Rabbit

Reactivity:

Human, Monkey

Applications:

WB, IF/IC, IP

Background:

Rabbit polyclonal antibody to Semaphorin 4A

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Semaphorin 4A. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of Semaphorin 4A expression in HeLa (A), COS7 (B) whole cell lysates.

IHC description:

IF/ICC description:

Immunofluorescent analysis of Semaphorin 4A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

Immunoprecipitation of Semaphorin 4A from 0.5mg Jurkat whole cell extract lysate, using 5ug of anti-Semaphorin 4A Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with anti-Semaphorin 4A Antibody.

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.