Clonality:

Polyclonal

Synonyms:

MGF; SCF; Kit ligand; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligand

Host:

Rabbit

Reactivity:

Human

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to SCF

Immunogen:

Recombinant full length protein of human SCF

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/100)

Conjugation:

WB description:

Western blot analysis of SCF expression in HT29 (A), HepG2 (B), Raji (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of SCF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.