Clonality:

Polyclonal

Synonyms:

40S ribosomal protein S6; Phosphoprotein NP33

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Chicken, Monkey, Sheep, Zebrafish

Applications:

WB, IF/IC

Background:

Rabbit polyclonal antibody to RPS6 (pS235)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RPS6 (pS235). The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Conjugation:

WB description:

Western blot analysis of RPS6 (pS235) expression in HeLa Calyculin A-treated (A), NIH3T3 (B) whole cell lysates.

IHC description:

IF/ICC description:

Immunofluorescent analysis of RPS6 (pS235) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.