Clonality:

Polyclonal

Synonyms:

1R20; BL34; IER1; Regulator of G-protein signaling 1; RGS1; B-cell activation protein BL34; Early response protein 1R20

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to RGS1

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RGS1. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of RGS1 expression in K562 (A), Raw264.7 (B), H9C2 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of RGS1 staining in human thymus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.