Clonality:

Polyclonal

Synonyms:

ATP6IP2; CAPER; ELDF10; Renin receptor; ATPase H(+)-transporting lysosomal accessory protein 2; ATPase H(+)-transporting lysosomal-interacting protein 2; ER-localized type I transmembrane adaptor; Embryonic liver differentiation factor 10; N14F; Renin/prorenin receptor; Vacuolar ATP synthase membrane sector-associated protein M8-9; ATP6M8-9; V-ATPase M8.9 subunit

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Chicken, Dog, Pig

Applications:

WB, IF/IC

Background:

Rabbit polyclonal antibody to Renin Receptor

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Renin Receptor. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Conjugation:

WB description:

Western blot analysis of Renin Receptor expression in HeLa (A), Raw264.7 (B), rat kidney (C) whole cell lysates.

IHC description:

IF/ICC description:

Immunofluorescent analysis of Renin Receptor staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.