Clonality:

Polyclonal

Synonyms:

C10orf28; GIDRP88; Coiled-coil domain-containing protein R3HCC1L; Growth inhibition and differentiation-related protein 88; Putative mitochondrial space protein 32.1; R3H and coiled-coil domain-containing protein 1-like

Host:

Rabbit

Reactivity:

Human, Mouse

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to R3HCC1L

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human R3HCC1L. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of R3HCC1L expression in HepG2 (A), Jurkat (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of R3HCC1L staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.