Clonality:

Polyclonal

Synonyms:

26S proteasome non-ATPase regulatory subunit 12; 26S proteasome regulatory subunit RPN5; 26S proteasome regulatory subunit p55

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to PSMD12

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PSMD12. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of PSMD12 expression in A431 (A), COLO320 (B), HeLa (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of PSMD12 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.