Clonality:

Polyclonal

Synonyms:

C14orf175; Phospholipase D4; PLD 4; Choline phosphatase 4; Phosphatidylcholine-hydrolyzing phospholipase D4

Host:

Rabbit

Reactivity:

Human, Mouse

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to Phospholipase D4

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Phospholipase D4. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of Phospholipase D4 expression in human tonsil (A), mouse testis (B), mouse muscle (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of Phospholipase D4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.