Clonality:

Polyclonal

Synonyms:

PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Pig, Rabbit, Sheep

Applications:

WB, IHC, IP

Background:

Rabbit polyclonal antibody to PDPK1 (pS241)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PDPK1. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of PDPK1 (pS241) expression in HeLa (A), MCF7 (B), NIH3T3 (C), PC12 EGF-treated (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of PDPK1 (pS241) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.