Clonality:

Polyclonal

Synonyms:

KIAA1649; PDIP46; Polymerase delta-interacting protein 3; 46 kDa DNA polymerase delta interaction protein; p46; S6K1 Aly/REF-like target; SKAR

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to p46

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p46. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/50 - 1/100)

Conjugation:

WB description:

Western blot analysis of p46 expression in PC12 (A), CT26 (B), Hela (C), MCF7 (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of p46 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.