Clonality:

Polyclonal

Synonyms:

CDKN2; MTS1; Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3; Cyclin-dependent kinase 4 inhibitor A; CDK4I; Multiple tumor suppressor 1; MTS-1; p16-INK4a; p16-INK4; p16INK4A

Host:

Rabbit

Reactivity:

Human

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to p16 INK4a (pS326)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p16 INK4a (pS326). The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of p16 INK4a (pS326) expression in HeLa UV-treated (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of p16 INK4a (pS326) staining in human cervix cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.