Clonality:

Polyclonal

Synonyms:

HNL; NGAL; Neutrophil gelatinase-associated lipocalin; NGAL; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC, IF/IC

Background:

Rabbit polyclonal antibody to NGAL

Immunogen:

KLH-conjugated synthetic peptide of human NGAL

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of NGAL expression in SW480 (A), A549 (B) whole cell lysates.

IHC description:

IF/ICC description:

Immunofluorescent analysis of NGAL staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.