Clonality:

Polyclonal

Synonyms:

NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; NEDD-8; Ubiquitin-like protein Nedd8

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Dog, Rabbit

Applications:

WB, IHC, IP

Background:

Rabbit polyclonal antibody to NEDD8

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NEDD8. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of NEDD8 expression in BJAB (A), K562 (B), HeLa (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of NEDD8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.