Clonality:

Polyclonal

Synonyms:

MOB4A; MOBKL1A; MOB kinase activator 1B; Mob1 homolog 1A; Mob1A; Mob1B; Mps one binder kinase activator-like 1A

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Monkey, Zebrafish

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to MOB1B

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MOB1B. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of MOB1B expression in Ramos (A), HEK293T (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of MOB1B staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.