Clonality:

Polyclonal

Synonyms:

BHLHE32; Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32; bHLHe32

Host:

Rabbit

Reactivity:

Human, Mouse

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to MITF (pS180)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MITF. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/50 - 1/100)

Conjugation:

WB description:

Western blot analysis of MITF (pS180) expression in U87MG (A), A549 (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of MITF (pS180) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.