Clonality:

Polyclonal

Synonyms:

Low-density lipoprotein receptor-related protein 11; LRP-11

Host:

Rabbit

Reactivity:

Human

Applications:

WB, IP

Background:

Rabbit polyclonal antibody to LRP11

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LRP11. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of LRP11 expression in A431 (A), HepG2 (B), Jurkat (C) whole cell lysates.

IHC description:

IF/ICC description:

IP description:

Immunoprecipitation of LRP11 from 0.5mg Jurkat whole cell extract lysate, using 5ug of anti-LRP11 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with anti-LRP11 Antibody.

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.