Rabbit anti-LCK Antibody YLD4681
Specifications
| 50ul / 100ul price = 100ul |
Clonality:
Polyclonal
Synonyms:
Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
Host:
Rabbit
Reactivity:
Human, Mouse, Rat
Applications:
WB, IHC, IF/IC
Background:
Rabbit polyclonal antibody to LCK
Immunogen:
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LCK. The exact sequence is proprietary.
Purification method:
The antibody was purified by immunogen affinity chromatography.
Concentration:
1mg/mL
Buffer:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Conjugation:
WB description:
Western blot analysis of LCK expression in Hela (A), Jurkat (B), mouse kidney (C), rat liver (D) whole cell lysates.
IHC description:
Immunohistochemical analysis of LCK staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description:
Immunofluorescent analysis of LCK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IP description:
FC description:
ChIP description
Storage:
Store at -20℃. Avoid repeated freeze / thaw cycles.