Clonality:

Polyclonal

Synonyms:

NKSF1; Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1

Host:

Rabbit

Reactivity:

Human, Bovine, Dog, Pig, Sheep

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to IL-12A

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IL-12A. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of IL-12A expression in MDA-MB231 (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of IL-12A staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.