Clonality:

Polyclonal

Synonyms:

Interferon gamma; IFN-gamma; Immune interferon

Host:

Rabbit

Reactivity:

Human

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to IFN gamma

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IFN gamma. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of IFN gamma expression in MOLT4 (A), Jurkat (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of IFN gamma staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.