Clonality:

Polyclonal

Synonyms:

BZF1; IDOL; E3 ubiquitin-protein ligase MYLIP; Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein; MIR

Host:

Rabbit

Reactivity:

Human, Mouse, Bovine, Monkey

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to IDOL

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IDOL. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of IDOL expression in MCF7 (A), mouse heart (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of IDOL staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.