Clonality:

Polyclonal

Synonyms:

Hydroxymethylglutaryl-CoA lyase, mitochondrial; HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase

Host:

Rabbit

Reactivity:

Human, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to HMGCL

Immunogen:

Recombinant full length protein of human HMGCL

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of HMGCL expression in SKOV3 (A), MCF7 (B), mouse liver (C), mouse kidney (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of HMGCL staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.