Clonality:

Polyclonal

Synonyms:

H3FT; Histone H3.1t; H3/t; H3t; H3/g

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC, IP, ChIP

Background:

Rabbit polyclonal antibody to Histone H3

Immunogen:

KLH-conjugated synthetic peptide of human Histone H3

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200), IP (1/50 - 1/100), ChIP (1/100 - 1/500)

Conjugation:

WB description:

Western blot analysis of Histone H3 expression in A431 (A), Hela (B), mouse liver (C), rat kidney (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of Histone H3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.