Clonality:

Polyclonal

Synonyms:

Histone H2B type F-S; Histone H2B.s; H2B/s

Host:

Rabbit

Reactivity:

Human, Mouse, Bovine, Chicken, Monkey, Zebrafish

Applications:

WB, IHC, IF/IC, ChIP

Background:

Rabbit polyclonal antibody to Histone H2B

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H2B. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of Lamin A/C (pS392) expression in SHSY5Y (A), mouse heart (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of Lamin A/C (pS392) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

Immunofluorescent analysis of Lamin A/C (pS392) staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.