Clonality:

Polyclonal

Synonyms:

H2AX; Histone H2AX; H2a/x; Histone H2A.X

Host:

Rabbit

Reactivity:

Mouse, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to Histone H2A.X

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence of mouse Histone H2A.X. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/200 - 1/500)

Conjugation:

WB description:

Western blot analysis of Histone H2A.Z expression in rat brain (A), mouse brain (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of Histone H2A.Z staining in mouse spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.