Clonality:

Polyclonal

Synonyms:

HDAC5; KIAA0600; Histone deacetylase 5; HD5; Antigen NY-CO-9; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR; Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR

Host:

Rabbit

Reactivity:

Human, Mouse, Bovine, Chicken, Dog, Pig, Rabbit

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to Histone Deacetylase 5/9 (pS259/220)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Histone Deacetylase 5/9 (pS259/220). The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of Histone Deacetylase 5/9 (pS259/220) expression in PC3 (A), HeLa (B), NIH3T3 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of Histone Deacetylase 5/9 (pS259/220) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.