Clonality:

Polyclonal

Synonyms:

KIAA0032; Probable E3 ubiquitin-protein ligase HERC3; HECT domain and RCC1-like domain-containing protein 3

Host:

Rabbit

Reactivity:

Human, Mouse

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to Herc3

Immunogen:

Recombinant full length protein of human Herc3

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of Herc3 expression in A431 (A), mouse lung (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of Herc3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.