Clonality:

Polyclonal

Synonyms:

Growth factor receptor-bound protein 14; GRB14 adapter protein

Host:

Rabbit

Reactivity:

Human

Applications:

WB, IHC, IP

Background:

Rabbit polyclonal antibody to GRB14

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GRB14. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of GRB14 expression in MOLT4 (A), COLO205 (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of GRB14 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.