Clonality:

Monoclonal

Synonyms:

GRL; Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Rabbit, Sheep

Applications:

WB, IHC, IF/IC, IP

Background:

Rabbit polyclonal antibody to Glucocorticoid Receptor

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Glucocorticoid Receptor. The exact sequence is proprietary.

Purification method:

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/1000 - 1/2000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of HIF1 beta expression in mouse brain (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of HIF1 beta staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.