Clonality:

Polyclonal

Synonyms:

EMR1; TM7LN3; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; Adhesion G protein-coupled receptor E1

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to F4/80

Immunogen:

Recombinant protein of human F4/80. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Conjugation:

WB description:

Western blot analysis of F4/80 expression in Hela (A), mouse liver (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of F4/80 staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.