Clonality:

Polyclonal

Synonyms:

E2F4; Transcription factor E2F4; E2F-4; E2F5; Transcription factor E2F5; E2F-5

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Chicken

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to E2F4

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human E2F4. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of E2F4 expression in HeLa (A), K562 (B), Jurkat (C), Raji (D), A431 (E), mouse kidney (F) whole cell lysates.

IHC description:

Immunohistochemical analysis of E2F4 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.