Clonality:

Polyclonal

Synonyms:

Transcription factor E2F2; E2F-2

Host:

Rabbit

Reactivity:

Human

Applications:

WB, IHC, IP

Background:

Rabbit polyclonal antibody to E2F2

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human E2F2. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of E2F2 expression in A431 (A), Jurkat (B) whole cell lysates.

IHC description:

Immunohistochemical analysis of E2F2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.