Clonality:

Polyclonal

Synonyms:

MTPOLB; DNA polymerase subunit gamma-2, mitochondrial; DNA polymerase gamma accessory 55 kDa subunit; p55; Mitochondrial DNA polymerase accessory subunit; MtPolB; PolG-beta

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to DNA Polymerase gamma 2

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DNA Polymerase gamma 2. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of DNA Polymerase gamma 2 expression in A549 (A), HeLa (B), NS-1 (C), H9C2 (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of DNA Polymerase gamma 2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.