Clonality:

Polyclonal

Synonyms:

DBP5; DDX19; TDBP; ATP-dependent RNA helicase DDX19B; DEAD box RNA helicase DEAD5; DEAD box protein 19B

Host:

Rabbit

Reactivity:

Human

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to DDX19B

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DDX19B. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of DDX19B expression in HepG2 (A), K562 (B), A549 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of DDX19B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.