Clonality:

Polyclonal

Synonyms:

CX32; Gap junction beta-1 protein; Connexin-32; Cx32; GAP junction 28 kDa liver protein

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Dog, Pig, Monkey

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to Connexin 32

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Connexin 32. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of Connexin 32 expression in MCF7 (A) whole cell lysates.

IHC description:

Immunohistochemical analysis of Connexin 32 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.