Clonality:

Polyclonal

Synonyms:

CLH17; CLTCL2; KIAA0034; Clathrin heavy chain 1; Clathrin heavy chain on chromosome 17; CLH-17

Host:

Rabbit

Reactivity:

Human, Mouse, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to Clathrin Heavy Chain

Immunogen:

KLH-conjugated synthetic peptide of human Clathrin Heavy Chain

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/2000), IH (1/50 - 1/100)

Conjugation:

WB description:

Western blot analysis of Clathrin Heavy Chain expression in Hela (A), MCF7 (B), mouse lung (C), rat brain (D) whole cell lysates.

IHC description:

Immunohistochemical analysis of Clathrin Heavy Chain staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.