Clonality:

Polyclonal

Synonyms:

CDC37A; Hsp90 co-chaperone Cdc37; Hsp90 chaperone protein kinase-targeting subunit; p50Cdc37

Host:

Rabbit

Reactivity:

Human, Mouse, Rat, Bovine, Chicken

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to CDC37 (pS13)

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDC37 (pS13). The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of CDC37 (pS13) expression in HuvEc (A), K562 (B), NIH3T3 (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of CDC37 (pS13) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.