Clonality:

Polyclonal

Synonyms:

ANAPC3; D0S1430E; D17S978E; Cell division cycle protein 27 homolog; Anaphase-promoting complex subunit 3; APC3; CDC27 homolog; CDC27Hs; H-NUC

Host:

Rabbit

Reactivity:

Human, Bovine

Applications:

WB, IHC, IP

Background:

Rabbit polyclonal antibody to CDC27

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC27. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Conjugation:

WB description:

Western blot analysis of CDC27 expression in A549 (A), K562 (B), Jurkat (C), HEK293T (D), HeLa (E) whole cell lysates.

IHC description:

Immunohistochemical analysis of CDC27 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.