Clonality:

Polyclonal

Synonyms:

ATP synthase subunit d, mitochondrial; ATPase subunit d

Host:

Rabbit

Reactivity:

Human, Rat

Applications:

WB, IHC

Background:

Rabbit polyclonal antibody to ATP5H

Immunogen:

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ATP5H. The exact sequence is proprietary.

Purification method:

The antibody was purified by immunogen affinity chromatography.

Concentration:

1mg/mL

Buffer:

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution:

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Conjugation:

WB description:

Western blot analysis of ATP5H expression in Hela (A), SHSY5Y (B), Rat muscle (C) whole cell lysates.

IHC description:

Immunohistochemical analysis of ATP5H staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description:

IP description:

FC description:

ChIP description

Storage:

Store at -20℃. Avoid repeated freeze / thaw cycles.