Description: Molecular cloning assisted by vectors is the most popular and common method to obtain genes of interest. Yeastern Biotech’s T&A™ Cloning Kit offers a quick, reliable and efficient method for cloning a variety of DNA sequences.
The T&A™ Cloning Kit contains the T&A™ Cloning Vector and all the reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq DNA polymerase, which add a single terminal 3′-dA nucleotide overhang. After ligation, the mixture can be used directly for transformation into competent cells (ECOS™) or be purified first to achieve higher transformation efficiency.
Features: Fast ligation (completed in only 5 min.), High transformation efficiency, More accurate results, Accept a wide range of inserts with different sizes, Two types of ligation buffers provided for your convenience, Allow blue/white screening, Contain ampicillin marker for antibiotic selection, Include M13 primer sites for convenient sequencing.
Applications:
- Cloning of terminal 3’-dA nucleotides overhang PCR products up to 5 kb
Quality Control
- DNA concentration of T&A™ Cloning Vector is 25 ng/μl
- The absorbance ratio (A260/A280) is between 1.6~2.0
- The size of T&A™ Cloning Vector is about 2.7 kb
- The colony number of background control is less than 50 when the transformation efficiency of competent cells is 1 × 108cfu/μg DNA
- The colony number ratio of self-ligation control to positive control is less than 15%
- The colony number of the positive control is more than 500 when the transformation efficiency of competent cells is 5 × 108cfu/μg DNA
- The ligation correctness with the control insert into T&A™ Cloning Vector is more than 87.5%