Product overview:

Carrier-free (BSA/glycerol-free) BFGF mouse monoclonal antibody, clone LBI3D10

Field of research:

MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton

Summary:

BFGF is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Alternative names:

BFGF; FGF-2; FGFB; HBGF-2

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

FGF2

Isotype:

IgG2b

Immunogen:

Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.

Clone:

LBI3D10

Conjugation:

Unconjugated

Gene name:

Homo sapiens fibroblast growth factor 2 (FGF2), transcript variant 1, mRNA.

Symbol:

FGF2

Pathway:

MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton

Function:

Druggable Genome, Secreted Protein

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:1000, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com