Product overview:

Carrier-free (BSA/glycerol-free) CD274 mouse monoclonal antibody,clone LBI9E5

Field of research:

Cell adhesion molecules (CAMs)

Summary:

This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Alternative names:

B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1; PDCD1LG1; PDL1

Species reactivity:

Human

Host:

Mouse

Target:

CD274

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human CD274 (NP_054862) produced in HEK293T cell.

Clone:

LBI9E5

Conjugation:

Unconjugated

Gene name:

Homo sapiens CD274 molecule (CD274), transcript variant 1, mRNA.

Symbol:

CD274

Pathway:

Cell adhesion molecules (CAMs)

Function:

Druggable Genome, Transmembrane

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com