Product overview:

Carrier-free (BSA/glycerol-free) DUSP1 mouse monoclonal antibody,clone LBI5A8

Field of research:

MAPK signaling pathway

Summary:

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]

Alternative names:

CL100; HVH1; MKP-1; MKP1; PTPN10

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

DUSP1

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human DUSP1 (NP_004408) produced in E.coli.

Clone:

LBI5A8

Conjugation:

Unconjugated

Gene name:

Homo sapiens dual specificity phosphatase 1 (DUSP1), mRNA.

Symbol:

DUSP1

Pathway:

MAPK signaling pathway

Function:

Druggable Genome, Phosphatase

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com