Product overview:

Carrier-free (BSA/glycerol-free) DDX39B mouse monoclonal antibody,clone LBI2C12

Field of research:

Spliceosome

Summary:

Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly with [PubMed:23299939] reporting a stimulatory effect. [UniProtKB/Swiss-Prot Function]

Alternative names:

BAT1; D6S81E; UAP56

Species reactivity:

Human, Monkey, Mouse, Rat, Dog

Host:

Mouse

Target:

DDX39B

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 319-428 of human DDX39B (NP_004631) produced in E.coli.

Clone:

LBI2C12

Conjugation:

Unconjugated

Gene name:

Homo sapiens DExD-box helicase 39B (DDX39B), transcript variant 1, mRNA.

Symbol:

DDX39B

Pathway:

Spliceosome

Function:

Request info at info@sobekbio.com

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com