Product overview:

Carrier-free (BSA/glycerol-free) LDLR mouse monoclonal antibody,clone LBI7C5

Field of research:

Endocytosis

Summary:

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

Alternative names:

FH; FHC; FHCL1; LDLCQ2

Species reactivity:

Human

Host:

Mouse

Target:

LDLR

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human LDLR (NP_000518) produced in HEK293T cell.

Clone:

LBI7C5

Conjugation:

Unconjugated

Gene name:

Homo sapiens low density lipoprotein receptor (LDLR), transcript variant 1, mRNA.

Symbol:

LDLR

Pathway:

Endocytosis

Function:

Druggable Genome, ES Cell Differentiation/IPS, Transmembrane

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500~2000, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com