Product overview:

Carrier-free (BSA/glycerol-free) CDC25B mouse monoclonal antibody,clone LBI6H9

Field of research:

Cell cycle, MAPK signaling pathway, Progesterone-mediated oocyte maturation

Summary:

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]

Alternative names:

cell division cycle 25 homolog B (S. pombe); cell division cycle 25B; OTTHUMP00000030137; OTTHUMP00000030138; OTTHUMP00000030139

Species reactivity:

Human

Host:

Mouse

Target:

CDC25B

Isotype:

IgG2b

Immunogen:

Human recombinant protein fragment corresponding to amino acids 421-567 of human CDC25B (NP_004349) produced in E.coli.

Clone:

LBI6H9

Conjugation:

Unconjugated

Gene name:

Homo sapiens cell division cycle 25B (CDC25B), transcript variant 2, mRNA.

Symbol:

CDC25B

Pathway:

Cell cycle, MAPK signaling pathway, Progesterone-mediated oocyte maturation

Function:

Druggable Genome, Phosphatase

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com