Product overview:

Carrier-free (BSA/glycerol-free) ATP5I mouse monoclonal antibody,clone LBI1G4

Field of research:

Metabolic pathways, Oxidative phosphorylation

Summary:

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Alternative names:

ATP5K

Species reactivity:

Human

Host:

Mouse

Target:

ATP5ME

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human ATP5I (NP_009031) produced in E.coli.

Clone:

LBI1G4

Conjugation:

Unconjugated

Gene name:

Homo sapiens ATP synthase membrane subunit e (ATP5ME), transcript variant 1, mRNA; nuclear gene for mitochondrial product.

Symbol:

ATP5ME

Pathway:

Metabolic pathways, Oxidative phosphorylation

Function:

Request info at info@sobekbio.com

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:500

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com